What is pGEX plasmid?
Plasmid: pGEX-1 Expression vector permitting production of a fusion protein. (ATCC staff) The glutathione S-transferase (GST) fusion protein can be purified by glutathione affinity chromatography, and the desired polypeptide released from the fusion product by thrombin.
What is an expression vector example?
An example of expression vector is the plasmid used to produce insulin important for treating diseases such as diabetes. Another example is the expression vector that introduces specific genes necessary for the synthesis of beta-carotene into the cells of rice plants, giving rise to a new variety called golden rice.
What is pGEX 2T?
Bacterial vector for expressing GST fusion proteins with a thrombin site.
What is in an expression vector?
An expression vector must have elements necessary for gene expression. These may include a promoter, the correct translation initiation sequence such as a ribosomal binding site and start codon, a termination codon, and a transcription termination sequence.
What are expression vectors PPT?
Expression Vectors: Vectors that can yield the protein products of the cloned genes. Two elements that are required for active gene expression: a strong promoter and a ribosome binding site near an initiating ATG codon.
What is shuttle vector example?
A vector that can replicate in more than one host organisms or two different cell types (e.g. a prokaryotic cell and a eukaryotic cell). An example is the yeast shuttle vector that can propagate within the cells of E. coli and yeast.
What is a pGEX vector?
pGEX-2TK is designed to allow the detection of expressed proteins by directly labeling the tagged products in vitro. This vector contains the recognition sequence for the catalytic subunit of cAMP-dependent protein kinase obtained from heart muscle.
What are shuttle and expression vectors?
Shuttle vector and expression vector are two types of vectors used in molecular biological experiments. A shuttle vector is usually a plasmid that is not designed for gene expression studies in cells, while an expression vector is usually a plasmid or virus that is designed for gene expression studies in cells.
What is Mcq shuttle vector?
Explanation: A shuttle vector is the one that can be used for transferring genetic material to more than one type of cells; essentially a eukaryotic and a prokaryotic system. 14. Yep13 is a shuttle vector. a) True.
What is pET plasmid?
The pET vector exists as a low copy number plasmid in host E. coli, which reduces leaky expression before induction. The vector utilizes the T7lac promoter system for strong and tightly controlled gene expression.
Is pUC19 an expression vector?
pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number.
What is the difference between pgex-6p1 and pgex-6p3?
The last number refers to the reading frame of the vector. pGEX-6P3 has an additional base in the multiple cloning site compared with pGEX-6P2, which in turn has an additional base compared with pGEX-6P1. Multiple reading frames enable you to select the appropriate vector for your application to avoid frameshift.
How many pGEX vectors are available?
Thirteen pGEX vectors are available (see Figure). Nine of the vectors have an expanded multiple cloning site (MCS) that contains six restriction sites.
How are pGEX vectors engineered with a lacIq gene?
All pGEX vectors are also engineered with an internal lacIq gene. The lacIq gene product is a repressor protein that binds to the operator region of the tac promoter, preventing expression until induction by IPTG, thus maintaining tight control over expression of the insert.
What is the E coli BL21 code for the pGEX vector?
Simplified package with only the pGEX vector in the pack ( E. coli BL21 will continue to be available separately, code no 27-1542-01. Updated Product Specification sheets.