What is mKate2?
mKate2 is a basic (constitutively fluorescent) red fluorescent protein published in 2009, derived from Entacmaea quadricolor. It is reported to be a rapidly-maturing monomer with moderate acid sensitivity.
What is Photoconvertible fluorescent protein?
Photoconvertible fluorescent proteins (pcFPs) constitute a large group of fluorescent proteins related to green fluorescent protein (GFP) that, when exposed to blue light, bear the capability of irreversibly switching their emission color from green to red.
What is mNeonGreen fluorescent protein?
mNeonGreen is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 2013, derived from Branchiostoma lanceolatum. It is reported to be a very rapidly-maturing monomer with moderate acid sensitivity.
What is Photoswitchable fluorescent protein?
Reversibly photoswitchable fluorescent proteins (RSFPs) are fluorescent proteins whose fluorescence, upon excitation at a certain wavelength, can be switched on or off by light in a reversible manner.
How are fluorescent proteins used?
Photoactivatable fluorescent proteins enable tracking of photolabeled molecules and cells in space and time and can also be used for super-resolution imaging. Genetically encoded sensors make it possible to monitor the activity of enzymes and the concentrations of various analytes.
Is mCherry far red?
mCherry is an excellent fluorescent protein that has maximal emittance in the red spectral range and a high fluorescence quantum yield, and thus, can be used as a donor for energy transfer to a far-red acceptor, such as BDFP1.
How does Photoactivatable fluorescent protein work?
Photoactivatable fluorescent proteins (PA-FPs) are fluorescent proteins that display unique changes in their spectral properties upon exposure to a specific wavelength of light.
How does red fluorescent protein work?
Red fluorescent protein (RFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP can be excited by the 488 nm or 532 nm laser line and is optimally detected at 588 nm.
What is the function of mNeonGreen?
mNeonGreen worked very well to tag proteins, label specific subcellular compartments, and report expression patterns of low-expression genes. We conclude that mNeonGreen represents a valuable alternative to complement standard GFPs, and we have prepared a plasmid set for its dissemination in the research community.
What are Photoswitchable molecules?
Photoswitches are molecules that undergo structural changes in response to light irradiation. The molecules generally possess two stable isomeric forms that are under equilibrium and can be reversibly obtained by irradiation and thermal activation [1].
Why do we need fluorescent proteins?
What is fluorescence imaging used for?
Fluorescence imaging is a type of non-invasive imaging technique that can help visualize biological processes taking place in a living organism. Images can be produced from a variety of methods including: microscopy, imaging probes, and spectroscopy.