What is Diff-Quik used for?

What is Diff-Quik used for?

Diff-Quik is a commercial Romanowsky stain variant used to rapidly stain and differentiate a variety of pathology specimens. It is most frequently used for blood films and cytopathological smears, including fine needle aspirates.

How often should Diff-Quik be changed?

Good laboratory practice should document changing each Diff-Quik stain setup at regular intervals (for example, every week if there is an average of about five evaluations per week). For immediate evaluation on wet fixed samples, an immersion stain setup could pose some threat of cross-contamination.

What is a Thiazine stain?

Thiazine red staining is a reliable and rapid pos-mortem diagnostic tool for Alzheimer´s disease. Arch Neurocien.

How often should you change Diff-Quik?

Which stain is used for DIFF quick method?

“Diff-Quick” is a proprietory brand of a Romanowski stain. The Romanowski group of stains are defined as being the black precipitate formed from the addition of aqueous solutions of methylene blue and eosin, dissolved in methanol.

How do you dispose of Diff Quick stain?

Collect liquid in an appropriate container or absorb with an inert material (e.g. vermiculite, dry sand, earth), and place in a suitable container for reclamation or disposal. Do not use combustible materials, such as sawdust. Do not flush to sewer.

How do you make a Diff-Quik stain?

Method

1. Allow smears to dry.
2. Dip slide or tape-strip five times, for one second each, into Fixative.
3. Dip slide or tape-strip five times, for one second each, into Stain 1.
4. Dip slide or tape-strip five times, for one second each, into Stain 2.
5. Rinse slide or tape-strip in distilled water or Weise’s buffer, pH 7.2.

How do you remove a Diff Quick stain?

1. Air-dry the smear.
2. Fix in “Diff Quick” Fixative (or methanol) for 30 secs/drain.
3. Stain with “Diff Quick” solution II for 30 secs/drain.
4. Counterstain (optional) with “Diff Quick” solution I for 30 secs/drain.
5. Rinse in tap water to remove excess stain.
6. Rapidly dehydrate in absolute alcohol.
7. Clear and mount.