What happened to Santa Cruz Biotechnology?
On Friday the U.S. Department of Agriculture revoked the research license of Santa Cruz Biotechnology in a settlement over alleged Animal Welfare Act violations on the firm’s goat farm.
How do you dilute Santa Cruz antibodies?
We recommend a primary antibody starting dilution of 0.5-2.0 µg/ml. Wash membrane three times for 5 minutes each with TBST.
What does Santa Cruz Biotechnology do?
Santa Cruz Biotechnology is a world leader in the development of products for the biomedical research market. Over the past twenty years, the Company has focused on the ongoing development of research antibodies, biochemicals, labware and more recently has expanded into animal health care products.
Who owns Santa Cruz Biotechnology?
John and Brenda Stephenson
Santa Cruz Biotech is owned by John and Brenda Stephenson, who have spent the past fifteen years acquiring forty-four thousand hilly acres along California’s central coast, in hopes of restoring the historic San Juan Ranch to its former peak of nearly sixty thousand acres.
Is Santa Cruz Biotechnology legit?
Is Santa Cruz Biotechnology a good company to work for? Santa Cruz Biotechnology has an overall rating of 2.6 out of 5, based on over 202 reviews left anonymously by employees. 33% of employees would recommend working at Santa Cruz Biotechnology to a friend and 22% have a positive outlook for the business.
Where is Santa Cruz Biotechnology based?
Dallas, Texas
Santa Cruz Biotechnology headquarters are located in Dallas, Texas, with additional US facilities in Paso Robles, California, and Sun Valley, Idaho.
How do you dilute primary antibody for Western blot?
For western blots, incubate membrane with diluted primary antibody in either 5% w/v BSA or nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight. NOTE: Please refer to primary antibody product webpage for recommended primary antibody dilution buffer and recommended antibody dilution.
What happened to Santa Cruz antibodies?
The company has been hit with a $3.5 million dollar fine over its treatment of its goats and rabbits, and has to give up its license under the Animal Welfare Act. That means they can continue with mice, rats, and chickens, but the rabbit and goat antibody production is now shut down.
How much serum should I load on a Western blot?
once you quantify your crude samples (either serum or plasma) with a standard method (spectrophotometric or fluorometric), try to load around 20-30ug of the diluted sample, this should be an effective range to start with, always considering that also in defribinized samples (serum) albumin will make up to the 40-50% of …
Where is Santa Cruz Biotechnology?
Santa Cruz Biotechnology headquarters are located in Dallas, Texas, with additional US facilities in Paso Robles, California, and Sun Valley, Idaho.
How do you dilute protein for Western blotting?
Popular Answers (1) After determination of protein concentration, you should dilute your samples in same RIPA buffer (+ protease and phosphatase inh.). Then mix appropriate dilution in 1:1 to 2x Laemmli buffer (in our case, it’s from Sigma). In some cases, you can also use 4x Laemmli buffer.
What are the recommended protocols for Santa Cruz Biotechnology Products?
Santa Cruz Biotechnology, Inc. products, including antibodies, siRNAs, shRNAs, and CRISPR products, are optimized using these recommended protocols for optimal results. Recommended reagents are also featured in each protocol. 1. Western (Immuno-) Blotting
How do I use Cruz marker™ molecular weight standards with primary antibodies?
If Cruz Marker™ molecular weight standards ( sc-2035) are used in the gel and the primary antibody is a mouse IgG kappa light chain, mix the primary antibody with the unconjugated Cruz Marker™ MW Tag ( sc-516729) in either Blotto, or UltraCruz ® Blocking Reagent as appropriate.
How to dilute Cruz Marker™ mw tag-HRP?
If Cruz Marker™ molecular weight standards ( sc-2035) are used in the gel, mix the HRP-conjugated primary antibody with the Cruz Marker™ MW Tag-HRP ( sc-516732) in Blotto. Incubate membrane in this mixture for 2 hours at room temperature, with shaking. Optimal Cruz Marker™ MW Tag dilution range is 1:1000 to 1:2000.
Which IgG binding protein should I use for RGB western blotting?
For RGB Fluorescent or Indirect Near-Infrared (NIR) Western blotting detection, we highly recommend using our kappa or lambda chain mouse IgG binding proteins and a compatible imaging/detection system.