Where does mycoplasma contamination come from?
As mentioned previously, the main sources of mycoplasma contamination in a cell culture laboratory are animal-derived media products, laboratory personnel and cross contamination of other contaminated cell lines.
Can DMSO get contaminated?
Of course, DMSO can get contaminated. ANY TC (Tissue culture) work have to be done using aseptic technique and sterilized supplies. DMSO being toxic and its sterility is a separate issue. For instance, many of cancer treatments are toxic yet that does not mean it won’t get cells contaminated.
Does ATCC test for mycoplasma?
ATCC scientists use a proprietary PCR-based mycoplasma assay to test your cells. The assay quickly and reliably detects more than 60 species of mycoplasma, Acholeplasma, Spiroplasma, and Ureaplasma, including the top 8 species most likely to affect cell cultures: M. arginini, M.
What happens if cell culture is contaminated?
Characteristics in mammalian cell cultures: Bacterial contamination will usually cause sudden changes in pH. If a culture is contaminated with aerobic bacteria, then the medium will become acidic and appear yellow. Most cases of bacterial contamination in the cell culture laboratory are caused by aerobes.
What kills mycoplasma?
There are three classes of antibiotics that kill mycoplasma when used at relatively low concentrations: tetracyclines, macrolides and quinolones. Tetracyclines and macrolides block protein synthesis by interfering with ribosome translation, whereas quinolones inhibit replication of mycoplasma DNA.
How serious is mycoplasma contamination?
The contamination of cell cultures by mycoplasmas remains a major problem in cell culture. Mycoplasmas can produce a virtually unlimited variety of effects in the cultures they infect. These organisms are resistant to most antibiotics commonly employed in cell cultures.
Can bacteria grow in 100% DMSO?
Viable bacteria were found in six bottles of dimethyl sulfoxide (DMSO) at a concentration of approximately one bacterium per 4.4 mL. The 18 bacterial isolates appeared to be tolerating the DMSO rather than metabolizing it. DMSO must be assumed to be nonsterile unless it has been previously sterilized.
How do you sterilize DMSO?
Sterilization by aseptic filtration is the most commonly used method of sterilization for DMSO. It has been proven in the industry to be an acceptable sterilization method for solutions or liquids that may not be sterilized in a final container (5).
What is mycoplasma contamination?
How is mycoplasma treated?
What is the treatment for mycoplasma infection? Antibiotics such as erythromycin, clarithromycin or azithromycin are effective treatment. However, because mycoplasma infection usually resolves on its own, antibiotic treatment of mild symptoms is not always necessary.
What is culture contamination?
Cell culture contaminants can be divided into two main categories, chemical contaminants such as impurities in media, sera, and water, endotoxins, plasticizers, and detergents, and biological contaminants such as bacteria, molds, yeasts, viruses, mycoplasma, as well as cross contamination by other cell lines.
What is cultural contamination?
Cultural contamination was the alteration of a culture’s natural development by an outside influence or exposure to a more technologically advanced society. Cultural contamination could be sociological or technological and could have drastic consequences.
How are ATCC bacterial strain prepared?
Most ATCC bacterial strains for distribution are prepared as freeze-dried cultures. For the storage of freeze-dried organisms, ATCC uses both double-vialed glass ampoules and stoppered serum vials. Double-vialed glass ampoules are used for the lyophilization of batch cultures via a component freeze-dryer.
How are ATATCC bacterial cultures shipped and stored?
ATCC bacterial strains are shipped frozen on dry ice in plastic cryopreservation vials, as lyophilized cultures in glass ampoules or serum vials, or as live cultures on agar slants or in broth medium. Upon receipt of frozen cultures, immediately revive cultures by thawing and subsequently transferring cultures to an appropriate growth medium.
How to get started with an ATCC cell line?
Getting Started with an ATCC Cell Line 3. Cell Growth and Propagation 4. Complete Growth Media 5. Culture Vessels and Surfaces 6. Cryopreservation 7. Contamination and Biosafety
How does ATCC analyze bacterial samples for characteristics?
When bacterial strains are initially received by ATCC, they are analyzed for characteristic colony and bacterial morphology; this is observed via colony growth and Gram staining, respectively. Additionally, samples are tested for culture purity on blood agar plates.