What are fluorophores used for?
Fluorophores are sometimes used alone, as a tracer in fluids, as a dye for staining of certain structures, as a substrate of enzymes, or as a probe or indicator (when its fluorescence is affected by environmental aspects such as polarity or ions).
Who bought Molecular Probes?
CARLSBAD, CA — July 2, 2003 — Invitrogen Corporation (Nasdaq: IVGN) today announced that it has signed a definitive agreement to acquire Molecular Probes, Inc. in an all cash transaction for approximately $325 million.
How do you pick fluorophores?
Selecting the Right Fluorophores for Flow Cytometry Experiments
- Understand your flow cytometer.
- Consider target abundance.
- Research fluorophore properties.
- Increase panel size with tandem dyes.
- Think about using calibration and compensation beads.
- Never underestimate the importance of compensation controls.
How do you choose fluorophores for immunofluorescence?
Select fluorophores with high extinction coefficients ( ε ) One defining factor of a fluorophore’s brightness is its extinction coefficient (a measurement of the probability of absorbing a photon of light); the higher the value of the extinction coefficient the brighter the fluorophore.
Are fluorophores antibodies?
Fluorophores (or fluorochromes) are commonly used in conjugation with antibodies as detection reagents in applications such as flow cytometry. Fluorophores can absorb and emit light within a range of wavelengths, normally referred to as the absorbance (excitation) and emission spectra.
What are fluorophores made of?
Biological fluorophores are commonly comprised of fluorescent proteins such as GFP. They have been used for cell labeling and characterization with varied rates of success. Samples stained with GFP emit bright green fluorescent signals when excited with ultraviolet incident light.
What is a DNA probe used for?
A probe is a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a sample genome. The probe is placed into contact with the sample under conditions that allow the probe sequence to hybridize with its complementary sequence.
What are molecular probes how are they prepared?
Preparation of probes for hybridization involves in vitro incorporation of reporter molecules into nuclei acids. These reporters can be incorporated at one or both ends of nucleic acid molecules, giving specific, low density labeled probes.
Why are fluorophores conjugated?
Is PE a bright fluorophore?
PE (Ex-Max 496 nm/Em-Max 578 nm): R-phycoerythrin – is a pigment naturally found in red algae where it transfers light energy to chlorophyll during photosynthesis. PE has one of the largest absorption co-efficiencies making it one of the brightest fluorochromes available to date.
How are fluorophores produced?
A fluorophore is an organic molecule with the ability to absorb light at a particular wavelength and then emit it at a higher wavelength. To achieve this, photons of light from an excitation source are absorbed by the fluorophore’s electrons, raising their energy level and causing them to move to an excited state.
What is Invitrogen Molecular Probes fluorescence?
Invitrogen Molecular Probes fluorescence reagents are among the most peer-referenced in all of life science research.
What is a bright fluorophore?
This family of bright fluorophores enables the use of more markers off the violet channel and increases the detection of low-abundance antigens due to their super bright signals.
Which fluorophore should I choose for my experiment?
Which fluorophore you choose depends on the experiment you are doing and the instrument that you will use to measure the signal. find more detailed information on the fluorophore and its use by clicking on a hyperlinked dye name below.
What are super bright polymer dye fluorophores?
Invitrogen eBioscience Super Bright polymer dyes are violet laser–excitable fluorophores that expand the capabilities of multi-parametric flow cytometry analysis. This family of bright fluorophores enables the use of more markers off the violet channel and increases the detection of low-abundance antigens due to their super bright signals.